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Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer

Received: 7 November 2015     Accepted: 19 November 2015     Published: 1 February 2016
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Abstract

Biodegradation of mechanic workshop polluted soil amended with 2.4kg lime (Confluence fertilizer) was studied for a period of eight weeks (56 days). This was done by physicochemical analysis of the soil samples and microbiological analysis by spread plate inoculation on Nutrient agar (NA). The results revealed higher bacteria population in amended soil (AS) compared to oil free soil (OFS) and polluted soil (PS). The range of bacterial counts was Oil free soil (1.6×104 – 6.8 ×104 cfu/g), polluted soil (2.2×104 – 9.8×104 cfu/g) and Amended soil (1.62×104 – 1.12×105 cfu/g). The fungi counts range were Oil free soil (2.5×103 – 3.0×104 cfu/g), polluted soil (2.2×103 – 4.0×104 cfu/g) and Amended soil (2.1×103 – 2.7×103/sup> cfu/g). Eleven (11) organisms were isolated in the course of this study. The bacteria isolated were Pseudomonas putida, Bacillus subtilis, Micrococcus luteus, Proteus mirabilis and Staphylococcus aureus while the fungi isolated include Mucor mucedo, Candida tropicalis, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus and Rhizopus nigricans. The moisture content (MC), organic carbon, organic matter, pH and nitrate were higher in amended soil compared to unpolluted soil and polluted soil. The result of the study shows that lime (confluence fertilizer) can be used to enhanced biodegradation of oil polluted soil.

Published in International Journal of Environmental Monitoring and Analysis (Volume 4, Issue 1)
DOI 10.11648/j.ijema.20160401.14
Page(s) 21-26
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Lime, Polluted Soil, Biodegradation, Inoculation

References
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  • APA Style

    Stephen Emmanuel, Okwute Loretta Ojonoma, Idoko Peter Arome, Makolo Daniel. (2016). Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer. International Journal of Environmental Monitoring and Analysis, 4(1), 21-26. https://doi.org/10.11648/j.ijema.20160401.14

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    ACS Style

    Stephen Emmanuel; Okwute Loretta Ojonoma; Idoko Peter Arome; Makolo Daniel. Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer. Int. J. Environ. Monit. Anal. 2016, 4(1), 21-26. doi: 10.11648/j.ijema.20160401.14

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    AMA Style

    Stephen Emmanuel, Okwute Loretta Ojonoma, Idoko Peter Arome, Makolo Daniel. Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer. Int J Environ Monit Anal. 2016;4(1):21-26. doi: 10.11648/j.ijema.20160401.14

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  • @article{10.11648/j.ijema.20160401.14,
      author = {Stephen Emmanuel and Okwute Loretta Ojonoma and Idoko Peter Arome and Makolo Daniel},
      title = {Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer},
      journal = {International Journal of Environmental Monitoring and Analysis},
      volume = {4},
      number = {1},
      pages = {21-26},
      doi = {10.11648/j.ijema.20160401.14},
      url = {https://doi.org/10.11648/j.ijema.20160401.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijema.20160401.14},
      abstract = {Biodegradation of mechanic workshop polluted soil amended with 2.4kg lime (Confluence fertilizer) was studied for a period of eight weeks (56 days). This was done by physicochemical analysis of the soil samples and microbiological analysis by spread plate inoculation on Nutrient agar (NA). The results revealed higher bacteria population in amended soil (AS) compared to oil free soil (OFS) and polluted soil (PS). The range of bacterial counts was Oil free soil (1.6×104 – 6.8 ×104 cfu/g), polluted soil (2.2×104 – 9.8×104 cfu/g) and Amended soil (1.62×104 – 1.12×105 cfu/g). The fungi counts range were Oil free soil (2.5×103 – 3.0×104 cfu/g), polluted soil (2.2×103 – 4.0×104 cfu/g) and Amended soil (2.1×103 – 2.7×103/sup> cfu/g). Eleven (11) organisms were isolated in the course of this study. The bacteria isolated were Pseudomonas putida, Bacillus subtilis, Micrococcus luteus, Proteus mirabilis and Staphylococcus aureus while the fungi isolated include Mucor mucedo, Candida tropicalis, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus and Rhizopus nigricans. The moisture content (MC), organic carbon, organic matter, pH and nitrate were higher in amended soil compared to unpolluted soil and polluted soil. The result of the study shows that lime (confluence fertilizer) can be used to enhanced biodegradation of oil polluted soil.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer
    AU  - Stephen Emmanuel
    AU  - Okwute Loretta Ojonoma
    AU  - Idoko Peter Arome
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    DO  - 10.11648/j.ijema.20160401.14
    T2  - International Journal of Environmental Monitoring and Analysis
    JF  - International Journal of Environmental Monitoring and Analysis
    JO  - International Journal of Environmental Monitoring and Analysis
    SP  - 21
    EP  - 26
    PB  - Science Publishing Group
    SN  - 2328-7667
    UR  - https://doi.org/10.11648/j.ijema.20160401.14
    AB  - Biodegradation of mechanic workshop polluted soil amended with 2.4kg lime (Confluence fertilizer) was studied for a period of eight weeks (56 days). This was done by physicochemical analysis of the soil samples and microbiological analysis by spread plate inoculation on Nutrient agar (NA). The results revealed higher bacteria population in amended soil (AS) compared to oil free soil (OFS) and polluted soil (PS). The range of bacterial counts was Oil free soil (1.6×104 – 6.8 ×104 cfu/g), polluted soil (2.2×104 – 9.8×104 cfu/g) and Amended soil (1.62×104 – 1.12×105 cfu/g). The fungi counts range were Oil free soil (2.5×103 – 3.0×104 cfu/g), polluted soil (2.2×103 – 4.0×104 cfu/g) and Amended soil (2.1×103 – 2.7×103/sup> cfu/g). Eleven (11) organisms were isolated in the course of this study. The bacteria isolated were Pseudomonas putida, Bacillus subtilis, Micrococcus luteus, Proteus mirabilis and Staphylococcus aureus while the fungi isolated include Mucor mucedo, Candida tropicalis, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus and Rhizopus nigricans. The moisture content (MC), organic carbon, organic matter, pH and nitrate were higher in amended soil compared to unpolluted soil and polluted soil. The result of the study shows that lime (confluence fertilizer) can be used to enhanced biodegradation of oil polluted soil.
    VL  - 4
    IS  - 1
    ER  - 

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Author Information
  • Department of Microbiology, Kogi State University, Anyigba-Kogi State, Nigeria

  • Department of Microbiology, University of Abuja, Gwagwalada- Abuja, Nigeria

  • Department of Microbiology, Kogi State University, Anyigba-Kogi State, Nigeria

  • School of Preliminary Studies, Kogi State Polytechnic, Kogi State, Nigeria

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